The Seidel Test is used to assess the presence of anterior chamber leakage in the cornea. It is used as a screening test for many corneal disorders including corneal post-trauma, corneal perforation and corneal degeneration.
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The Seidel Test is named after the German Ophthalmologist Erich Seidel (1882–1948).
A fluorescein strip containing 10% fluorescein is applied topically to the affected area and is examined with a cobalt blue filter. At this point, the fluorescein appears dark orange in color. Any changes in color of the fluorescein strip indicate the presence of corneal deformities.
If the fluorescein strip turns pale upon application to the corneal surface, the person tests positive for the corneal deformity he/ she is being tested for. The change in the color of the fluorescein strip is due to dilution of fluorescein caused by the aqueous leakage in the cornea.
Do not apply pressure on the eye during the test due to the risk of tissue extrusion.